变应性鼻炎肾阳虚复合豚鼠模型筛选与评价

摘要

目的：在卵清蛋白致敏变应性鼻炎豚鼠模型基础上，采用糖皮质激素、羟基脲、腺嘌呤3种不同阳虚诱导剂诱发，建立变应性鼻炎肾阳虚复合模型，以筛选建立理想而稳定的变应性鼻炎（Allergic Rhinitis，AR）病证结合动物模型。方法：采用卵清蛋白（Ovalbumin，OVA）诱导建立变应性鼻炎豚鼠模型，同时给予糖皮质激素、羟基脲或腺嘌呤建立肾阳虚复合模型，观察动物宏观体征及死亡情况、局部过敏症状，酶联免疫吸附法（Enzyme-linked Immunosorbent Assay，ELISA）法测血清中 sIgE 和组胺含量，苏木精-伊红（Hematoxylin-eosin，HE）染色观察肾和睾丸组织形态学改变。结果：糖皮质激素、羟基脲和腺嘌呤3种致肾阳虚药物均可使动物表现出肾阳虚症状及体征、体重下降并具有一定的致死率。与空白组相比，糖皮质激素对肾脏和睾丸组织形态影响较小，羟基脲和腺嘌呤对肾脏和睾丸等形态结构影响较大。结论：对比3个肾阳虚复合模型，羟基脲复合组模型比激素复合组和腺嘌呤复合组模型更稳定，且死亡率较低，较为理想。不过变应性鼻炎病证结合动物模型的指标评价标准尚待进一步研究。%Objective:To establish complex models with allergic rhinitis of kidney-yang-deficiency by three different kidney-Yang-deficiency inducers,glucocorticoid,hydroxyurea and adenine,based on guinea pig models of allergic rhinitis sensitized by ovalbu-min,for screening a reliable and ideal model of allergic rhinitis of kidney yang deficiency pattern.Methods:Guinea pig models of Allergic Rhinitis sensitized by ovalbumin were set and glucocorticoid,hydroxyurea or adenine was used to build the complex mod-els of allergic rhinitis with kidney-yang deficiency.The macro signs,death and partial allergy symptoms of animals were observed, the serum sIgE and histamine were tested by ELISA (enzyme-linked immunosorbent assay)and the morphology change of kidney and testis tissue were observed by HE (hematoxylin-eosin)staining.Results:Three different Yang deficiency inducers,glucocor-ticoid,hydroxyurea and adenine,could lead to kidney-yang-deficiency symptoms and signs,weight loss,and cause certain death. Compared with the control group,it is found the affection on morphology of glucocorticoid to kidney and testis tissue was little, while which of hydroxyurea and adenine were great.Conclusion:By comparison of three different complex models with allergic rhinitis of kidney-yang deficiency,the model induced by hydroxyurea was more stable and suitable with lower death rate.More re-searches should be conducted for the evaluation criteria of the allergic rhinitis animal model.