“鼻敏爽”与姜黄素治疗豚鼠变应性鼻炎的筛选实验研究

摘要

目的：比较观察“鼻敏爽”与姜黄素治疗豚鼠变应性鼻炎的疗效差异。方法雄性豚鼠80只，卵清蛋白法制备变应性鼻炎模型，随机分为模型、强的松、姜黄素低中高剂量、鼻敏爽低中高剂量组，每组10只；另取10只豚鼠作为正常对照组，共计9组。分别应用“鼻敏爽”和姜黄素灌胃治疗，观察其行为学改变；检测豚鼠鼻黏膜以嗜酸性粒细胞浸润为主的炎症病理改变，血清组胺、IFN-γ、IL-2、IL-4及IL-5含量，比较分析组间差异。结果治疗2周后，强的松组和鼻敏爽高剂量组症状明显减轻，鼻黏膜炎症病变改善，血清组胺、IL-4及IL-5含量明显降低，IFN-γ及IL-2含量有所增高；姜黄素低、中、高剂量组及鼻敏爽低、中剂量组症状改善不明显，鼻黏膜炎症病变好转不明显，血清组胺、IFN-γ、IL-2、IL-4及IL-5含量与模型组相比无明显差异。结论强的松组和高剂量鼻敏爽治疗豚鼠变应性鼻炎有效，二者疗效相当，提示中药复方比单味药或单体更具有疗效优势。%Objective To investigate the therapeutic effect of Biminshuang and curcumine on guinea pigs with allergic rhinitis in a comparative way. Method Animal model of allergic rhinitis was prepared among 80 guinea pigs by the procedure of ovoalbumin as the sensitizer at first, and then, they were randomly divided into 8 groups, i.e. modeling group (MG), prednisone treating group (PTG), curcumine treating groups (CTG) treated at high, moderate or low dosage respectively (CTGh, CTGm, CTGl), and Biminshuang treating groups (BTG) treated at high, moderate or low dosage as well (BTGh, BTGm, BTGl), with another 10 guinea pigs taken as normal control group (NCG), 9 groups in total. They were treated with Biminshuang or curcumin according to the designed plan respectively and with their behavior observed during the whole experiment period. By the end of the treatment, all animals were put into death to collect samples of nasal mucosa and serum for pathohistological determination by HE stain and serum level detection of histamine, IFN-γ, IL-2, IL-4 and IL-5 by ELISA assay, with all these results comparatively analyzed to see their differences among different groups. Result By the end of therapy lasted for 2 weeks, symptom scores of PTG and BTGh were determined as significantly lowered, with markedly improved inflammatory lesion in nasal mucosa and obviously reduced levels of histamine, IL-4 and IL-5 in their serum, and a certain degree of serum level elevation of IFN-γ and IL-2 (P<0.05). However, all these indicators changed insignificantly among those animals in CTGh, CTGm, CTGl, BTGm and BTGl, when compared with that of MG (P>0.05). Conclusion Biminshuang at high dosage and prednisone are both effective in the treatment of experimental allergic rhinitis almost at a similar therapeutic level. This suggests that compound herbal medicine formulae holds more therapeutic advantages than one single herbal medicine or someone monomer element.