蜜炙酸枣仁中斯皮诺素含量测定

摘要

Objective:To determine the content of Spinosin in Honey-fried Semen Zizyphi Spinosae(Mizhi Suanzaoren)by HPLC (High Performance Liquid Chromatography).Methods:The experiment was conducted in the condition of WondaCract ODS-2(250 mm ×4.6 mm,5μm),mobile phase isacetonitrile-water,detection wavelength 335nm,and flow velocity 1 mL/min,column tempera-ture 30 ℃,using RP-HPLC.Results:The linearity range of Spinosin is 0.005 ~0.3 mg/mL,r =0.9965,average recovery rate is 99.74%,RSD =1.46%.Conclusion:This method is accurate with great reproducibility.It can offer testing standards for process-ing study and quality control of Honey-fried Semen Zizyphi Spinosae.%目的：以高效液相色谱法测定蜜炙酸枣仁中斯皮诺素的含量。方法：采用反相高效液相色谱法，以 WondaCract ODS-2（250 mm ×4.6 mm，5μm）为色谱柱，乙腈－水为流动相，检测波长335 nm，流速1 mL／min，柱温30℃。结果：斯皮诺素的线性范围0.005～0.3 mg／mL，r ＝0.9965，平均回收率为99.74％，RSD ＝1.46％。结论：该方法准确、重现性好，可为蜜炙酸枣仁的炮制工艺研究及质量控制提供检测标准。