不同纯化方法对丹参水提取液中丹酚酸B的含量影响

摘要

Objective:To explore the best purification method by observing effects of different purification ways on the extractive rate of salvianolic acid B in Salvia Miltiorrhiza Bge water-extraction.Methods:Salvianolic acid B content and dry extract yield rate were used as indexes for assessing the best purification method among ethanol precipitation,high speed centrifugation and macroporous resin absorption.Results:Among all three methods,salvianolic acid B content by macroporous absorption resin was the highest,with dry extract yield rate being the lowest;salvianolic acid B content by ethanol precipitation was the lowest,with dry extract yield rate being the medium level;and salvianolic acid B content by high speed centrifugation was at the medium level, with dry extract yield rate being the highest.Conclusion:Water extraction of Salvia miltiorrhiza Bge by macroporous absorption resin is considered as the best purification method.%目的：考察不同纯化方法对丹参水提液中丹酚酸 B 含量的影响，以优选出丹参水提取液的最佳纯化方法。方法：以丹酚酸 B 含量和浸膏得率为考察指标，对乙醇沉淀法、高速离心法和大孔树脂吸附法3种纯化方法进行了比较。结果：大孔树脂吸附法处理后所得到的丹酚酸 B 的含量最高，浸膏得率最低；乙醇沉淀法处理后丹酚酸 B 的含量最低，浸膏得率居中；高速离心法处理后丹酚酸 B 的含量居中，但浸膏得率最高。结论：大孔树脂吸附法为丹参水提液的最佳纯化方法。