'上火'人群中Activin A、E-selectin的差异表达研究

摘要

目的:通过对"上火"人群中细胞因子激活素A(ActivinA)及E选择素(E-selectin)血清含量的测定以及外周血单个核细胞基因表达水平检测,探讨激活素A及E选择素在"上火"人群发病机制的影响和作用.方法:采集临床患者血液样本并提取血清及外周血单个核细胞.应用酶联免疫吸附试验(ELISA)测定血清中激活素A及E选择素的含量,应用RT-PCR法检测激活素A及E选择素的基因表达水平.结果:"上火"患者血清中activinA、E-selectin的水平以及外周血单个核细胞activinA、E-selectin基因表达水平高于正常平和质人群;观察组外周血中activinA、E-selectin的基因表达水平高于阴虚"上火"人群.结论:"上火"是一种局部的炎性反应,机体内环境在多种因素的作用下激活素A水平升高,影响炎性反应介质的作用或者本身作为一种炎性反应介质活化血管内皮细胞刺激E选择素的分泌与合成,诱导炎性反应,这是"上火"机制之一.%Objective:To study of activin A and E selectin in the pathogenesis of "shanghuo" people and influence effect through the determination of serum content and the level of gene expression in peripheral blood mononuclear cells of activin A or E -selectin who suffered from excessive internal heat.Methods:Clinical lit crowd and gentleness in blood samples was collected and serum and peripheral blood mononuclear cells from blood samples was extracted.By enzyme-linked immune sorbent adsorption assay,the content of serum activin A and E-selectin were determined,and RT-PCR detection was sued to detect gene expression level of ac-tivin A and E-selectin.Results:The level of activin A and E-selectin in serum of patients with excessive internal heat was higher than that of normal and healthy people.The level of activin A and E-selectin gene expression in peripheral blood mononuclear cells of patients with internal heat was higher than that of normal and healthy people.The gene expression level of activin A and E-se-lectin in peripheral blood was higher than that of yin deficiency and heat syndrome.Conclusion:Shanghuo is a local inflammation.Under stress,activin A levels are elevated,effecting inflammatory mediators or itself as a mediator of inflammation activated vascu-lar endothelial cells stimulate E-selectin secretion and synthesis,induced inflammatory reaction and to cause the disorder of lipid metabolism in the body′s physiological balance,causing Shanghuo.