前列通片促进免疫功能的实验研究

摘要

Objective:To study the effect of Qianlietong Tablets in regulating immune function on mice.Methods:1) Splenic Lymphocyte transformation test:A total of 60 SPF grade BALB/c mice were randomly divided into negative control group,positive control group,and Qianlietong Tablets low-,medium-,and high-dose groups.After Intragastric administration of different medications for different groups for 30 days continuously,spleens of each group were obtained in aseptic condition.Then spleen lymphocytes were extracted for transformation test.2) NK cell activity effect test:A total of 60 SPF grade BALB/c mice were randomly divided into negative control group,model group,positive control group and Qianlietong Tablets low-,medium-,and high-dose groups.Except the negative group,mice model with low immune function was built in other groups by intraperitoneal injection of cyclophosphamide,and the mice models were given medicines as well.Mter 30 days of medication,spleens were obtained in aseptic condition.Spleen lymphocytes were extracted for testing influence on NK cell activity.Results:1) Compared with the negative control group,the spleen lymphocytes transformation ability of the Qianlietong Tablets high-dose group increased obviously with significant difference(P ＜0.05).2) Compared with the model group,the NK cell activity of the positive control group and the Qianlietong Tablets high-dose group increased obviously with statistical difference (P ＜ 0.05).Conclusion:Under the experimental condition,Qianlietong Tablets can improve lymphocytes transformation ability and NK cell activity,which proves that Qianlietong Tablets has the effect of promoting immune function.%目的:探讨前列通片对小鼠免疫功能的影响.方法:1)脾淋巴细胞转化试验:将60只小鼠随机分为阴性对照组、阳性对照组、前列通片低、中、高剂量组,每组12只.灌胃给药,1次/d,连续给药30 d后各组动物无菌取脾,提取脾淋巴细胞进行脾淋巴细胞转化试验.2)NK细胞活性影响试验:将60只小鼠随机分为阴性对照组、模型对照组、阳性对照组、前列通片低、中、高剂量组.除阴性对照组外,其余各组腹腔注射环磷酰胺建立免疫功能低下的小鼠模型.造模同时给药,连续给药30 d后,各组动物无菌取脾,提取脾淋巴细胞进行NK细胞活性影响试验.结果:1)与阴性对照组比较,前列通片高剂量组淋巴细胞转化能力显著升高,差异具有统计学意义(P＜0.05).2)与模型对照组比较,阳性对照组及前列通片高剂量组NK细胞活性显著升高,差异具有统计学意义(P＜0.05).结论:在本实验条件下,前列通片能够提高机体淋巴细胞转化能力以及NK细胞的活性,具有一定的免疫促进作用.