刺络泻血对高脂血症模型大鼠血脂、NO及ET-1影响的研究

摘要

Objective:To observe the effect of blood-letting puncture therapy on NO, ET-1 and blood lipid in rats with hyperlipidemia, and explore its therapeutic effect and mechanism in treating hyperlipidemia.Methods:A total of 32 male SD rats were randomly divided into 4 groups, which are control group, model group, blood pricking group and medical group.Except for the control group, the other three groups were made into hyperlipidemia models by high fat diet.After modeling, rats in control group received no treatment.The model group was fixed to receive blood-letting puncture therapy on the superficial vein of the rats hind leg, 0.3-0.5 mL each time, 2 times a week.To observe the levels of TC, TG, HDL-C, LDL-C, NO and ET-1. Results:Comparing to the control group, the level of TC, TG, LDL-C, ET-1 of the model groups significantly increased, and the level of NO content in the model groups significantly decreased with significant difference (P<0.01).Comparing to the control groups, the levels of TC, TG, LDL-C, ET-1 content in the other groups decrease, and the levels of NO content in the blood-letting group and medicine group increased, and the difference is significant (P<0.05,P<0.01).The content of HDL-C in each group do not have significant difference (P>0.05), however, compared to control group, the model group shows a tendency of decrease, while blood-letting group and medicine group has a tendency of increase.Conclusion:Blood-letting puncture therapy can correct lipid metabolism abnormalities.Besides, by inhibiting the increase of ET-1 content and maintain vascular endothelial cell balance, it can reduce and delay endothelial cell injury.%目的:观察刺络泻血疗法对高脂血症大鼠一氧化氮(NO)、内皮素-1(ET-1)及血脂的影响,探讨刺络泻血疗法对高脂血症模型大鼠调脂的作用及机制.方法:将32只雄性SD大鼠随机分为4组,每组8只即空白对照组、模型组、刺血观察组、药物观察组,除空白对照组外,其他组大鼠给予高脂饲料喂养建立高脂血症模型.造模成功后空白对照组不进行任何干预;模型组只进行抓取及按压;刺血观察组在大鼠后肢浅静脉进行刺血干预,0.3~0.5 mL/次,2次/周,治疗4周;药物观察组给予辛伐他丁2 mg/(kg·d)灌胃干预,治疗4周.观察大鼠的血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、一氧化氮(NO)、内皮素-1(ET-1)水平.结果:与空白组比较,模型组大鼠血清TC、TG、HDL-C、ET-1含量均显著上升,NO含量显著降低,差异有统计学意义(P<0.01);与模型组比较,刺血组及药物组大鼠血清TC、TG、HDL-C、ET-1含量均降低,NO含量均上升,差异有统计学意义(P<0.01);各组大鼠血清HDL-C含量差异无统计学意义(P>0.05),但模型组较空白组有下降趋势,刺血组及药物组较模型组均有上升趋势.结论:刺络泻血疗法可以纠正脂质代谢异常,并通过抑制ET-1含量的增加而维持血管内皮细胞平衡,从而减轻和延缓内皮细胞损伤.