鲜生地黄对慢加急性肝衰竭大鼠JAK2/STAT3通路的影响

摘要

目的:观察鲜生地黄对慢加急性肝衰竭大鼠JAK2/STAT3通路的影响.方法:30只雄性Wistar大鼠按照1∶4比例随机分为对照组和实验组.实验组大鼠先用四氯化碳诱导成肝纤维化模型,再按照1∶1比例随机分为模型组和观察组,应用D氨基半乳糖进行急性攻击,建立慢加急性肝衰竭大鼠模型.观察组在急性攻击前7d予以鲜生地黄连续灌胃.各组大鼠分别在急性攻击后24 h取材,检测血清内毒素、白细胞介素-6含量及肝脏组织中JAK2mRNA、STAT3mRNA的表达.结果:模型组大鼠肝组织中JAK2mRNA、STAT3mRNA表达低于对照组和观察组,差异有统计学意义(P＜0.05).观察组与对照组大鼠肝组织中JAK2mRNA、STAT3mRNA表达差异无统计学意义(P＞0.05).模型组大鼠血清内毒素显著高于对照组和观察组,差异有统计学意义(P＜0.01);观察组与对照组比较,大鼠血清内毒素表达差异无统计学意义(P＞0.05).模型组大鼠血清白细胞介素-6高于对照组和观察组,但差异无统计学意义(P＞0.05).结论:鲜生地黄能够降低肠道内毒素的吸收,促进JAK2/STAT3通路活化,但是介导物质可能并不是IL6,相关机制需要进一步完善.%Objective:To observe the effect of fresh Radix Rehmanniae Recens on JAK 2/STAT3 pathway in acute-on-chronic liver failure rats.Methods:Thirty male Wistar rats were randomly divided into control group and experimental group according to 1∶4 ra-tio.Rats in experimental group were firstly induced to hepatic fibrosis model with carbon tetrachloride ,and then randomly divided into model group and treatment group according to the ratio of 1∶1.Acute attacks were performed with D-galactosamine and a rat model of chronic acute liver failure was established.The treatment group was given intragastrically with the fresh Radix Rehmanniae Recens 7 days before the acute attack.Rats in each group were harvested 24 hours after acute challenge.Serum endotoxin,interleu-kin-6 levels,and expression of JAK2 mRNA and STAT3 mRNA in liver tissue were measured.Results:The expression of JAK2 mRNA and STAT3 mRNA in the liver tissue of the model group was lower than that in the control group and the model group .The difference was statistically significant (P＜0.05).There was no significant difference in the expression of JAK 2 mRNA and STAT3 mRNA between the treatment group and the control group (P＞0.05).The serum endotoxin level in the model group was obviously higher than that in the control group and the treatment group ,and the difference was statistically significant (P＜0.01).There was no significant difference in serum endotoxin expression between the treatment group and the control group (P＞0.05).Serum inter-leukin-6 levels in the model group were higher than those in the control group and the treatment group ,but the difference was not statistically significant (P＞0.05).Conclusion:Fresh Radix Rehmanniae Recens can reduce the absorption of toxins in the intes-tine and promote the activation of JAK2/STAT3 pathway,but the mediator may not be IL6,and the relevant mechanisms need to be further improved.