陕产瞿麦中总黄酮提取工艺响应曲面法的优化

摘要

目的:评价响应曲面法优选出超声辅助提取陕产瞿麦中总黄酮类化合物的最优方案.方法:实验以陕产瞿麦为原料,乙醇为提取剂,在单因素实验的基础上,通过响应面实验对超声辅助法提取陕产瞿麦中总黄酮的工艺进行优化.结果:优选出了超声辅助提取陕产瞿麦中总黄酮的最佳工艺条件:液料比10倍、提取功率140 W、乙醇浓度80%时,总黄酮的提取率最大可以达到0.851%.结论:工艺的实验值与预测值基本相符,采用响应曲面法优选超声辅助提取陕产瞿麦中黄酮类化合物的提取工艺稳定可行,为陕产瞿麦资源的开发和利用提供一定的参考依据.%Objective:To select the optimized plan of ultrasonic assisted extracting total flavonoids in Herba Dianthi from Shaanxi by the response surface method.Methods:In this experiment, Shaanxi producing Herba Dianthi was taken as raw material , and ethanol as extraction agent.On the basis of single factor experiment , the extraction process of total flavonoids from Herba Dianthi of Shaanxi by ultrasonic assisted method was optimized with the response surface method .Results:The ultrasonic assisted extraction conditions of total flavonoids in Herba Dianthi from Shaanxi were optimized :liquid and material ratio was 10 times, extraction pow－er 140W, 80%ethanol concentration , and the extraction rate of flavonoids could reach 0.851%.Conclusion:The process of the experimental values are basically consistent with the predicted value , indicating using response surface methodology to optimize ul－trasonic assisted extraction technology of flavonoids in Herba Dianthi in Shaanxi Province is stable and feasible , and providing ref－erence for the development and utilization of Herba dianthi resources in Shaanxi Province.