甘肃当归对乙酰苯肼溶血性血虚小鼠模型的干预作用

摘要

Objective: To compare the intervention of DangGui (Angelica sinensis) of Minxian, Lixian, Weiyuan, Linxia of Gansu province on mice models with acetyl phenyl hydrazine-induced hemolytic blood-deficiency. Methods: The model with acetyl phenyl hydrazine-induced hemolytic blood-deficiency was established and given DangGui water decoction [1.56 g/(kg·d)] for 22 days, to detect red blood cells (RBC), hemoglobin (HGB), hemat-ocrit (HCT), white blood cells (WBC), platelet (PLT), the activity of Na+-K+-ATP enzyme and Ca2+-Mg2+-ATP en-zyme in red cell membrane, and observe the changes of liver index (LI), spleen index (SI) and thymus index (TI). Results:Compared with the model group, Minxian Danggui could raise RBC, HGB and HCT but decrease WBC and PLT (P<0.05), Linxia Danggui could increase HGB and HCT but lower WBC and PLT (P<0.05), Weiyuan Dang-gui could increase HGB and HCT, but decrease PLT (P<0.05), Lixian Danggui could decrease PLT (P<0.05);Minxian Danggui could improve the activity of Na+-K+-ATP enzyme and Ca2+-Mg2+-ATP enzyme (P<0.05), Lixian Danggui could improve the activity of Na+-K+-ATP enzyme (P<0.05), Linxia Danggui could improve the activity of Ca2+-Mg2+-ATP enzyme (P<0.05);Gansu Danggui could lower LI (P<0.05), showing the tendency of decreasing SI, Minxian, Lixian and Weiyuan Danggui could increase TI (P<0.05). Conclusion:Gansu Danggui [1.56 g/ (kg·d)] could improve the related indexes in peripheral blood of mice with acetyl phenyl hydrazine-induced hemolytic blood-deficiency obviously, improve the activity of ATP in red blood cell membrane eividently, decrease liver and spleen indexes and increase TI, and Minxian Danggui shows the strongest function comprehensively.%目的：比较甘肃岷县、礼县、渭源、临夏不同产地当归对乙酰苯肼溶血性血虚小鼠的干预作用。方法：建立乙酰苯肼溶血性血虚模型，给予当归水煎液[1.56 g/(kg·d）]22天后，检测外周血红细胞（RBC）、血红蛋白（HGB）、红细胞压积（HCT）、白细胞（WBC）、血小板（PLT）、红细胞膜钠-钾-ATP酶（Na+-K+-ATP）和钙-镁-ATP酶（Ca2+-Mg2+-ATP）活性，并观察肝脏指数（LI）、脾脏指数（SI）、胸腺指数（TI）的变化。结果：与模型组比较，岷县当归能提高RBC、HGB、HCT降低WBC、PLT（P＜0.05），临夏当归能提高HGB、HCT降低WBC、PLT（P＜0.05），渭源当归能提高HGB、HCT降低PLT （P＜0.05），礼县当归能降低PLT （P＜0.05）；岷县当归能提高Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性（P＜0.05），礼县当归能提高Na+-K+-ATP酶活性（P＜0.05），临夏当归能提高Ca2+-Mg2+-ATP酶活性（P＜0.05）；甘肃产各当归均能降低LI（P＜0.05），有降低SI的趋势，岷县、礼县、渭源当归能升高TI （P＜0.05）。结论：甘肃当归[1.56 g/(kg·d）]具有明显改善乙酰苯肼溶血性血虚小鼠外周血象相关指标，提高红细胞膜ATP活性，降低肝脾指数，升高胸腺指数的作用，综合比较岷县当归作用较强。