黑逍遥散对阿尔茨海默病模型大鼠行为学及血清肿瘤坏死因子α及白细胞介素-6和-1β的影响

摘要

Objective:To observe the effect of HeiXiaoYao powder on the rats with Alzheimer disease (AD) induced by Aβ 25-35 in the expression of tumor necrosis factor (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in blood serum and to study the functional mechanism of HeiXiaoYao powder in the prevention and treatment of AD. Methods: After the establishment of the AD rat models by injecting Aβ 25-35 in the hippocampus, the models were treated by HeiXiaoYao powder and huperzine A. On the 28th day, the contents of TNF-α, IL-6 and IL-1β in the serum of the models were exam ined by enzyme-linked immunosorbent assay (ELISA). Results:The TNF-α, IL-6 and IL-1βcontents of the control group were increased hugely, while in the treatment group the con-tents of TNF-α, IL-6 and IL-1βwere all decreased and the HeiXiaoYao powder group of high dose were significantly decreased. Conclusion:The HeiXiaoYao powder can effectively control the expression of the inflammatory factor for AD patients, which is one of important mechanisms of HeiXiaoYao powder.%目的：观察黑逍遥散对β-淀粉样蛋白（Aβ25-35）所致阿尔茨海默病（Alzheimer disease,AD）模型大鼠血清肿瘤坏死因子（TNF-α）、白细胞介素（IL-6、IL-1β）表达的影响，探讨黑逍遥散防治AD的作用机制。方法：海马区注射Aβ25-35建立AD大鼠模型，用黑逍遥散、石杉碱甲进行对抗治疗，第28天取血清，通过酶联免疫吸附法（ELISA）测定AD大鼠血清TNF-α、IL-6、IL-1β含量。结果：模型组大鼠血清中TNF-α、IL-6、IL-1β含量显著升高，各治疗组大鼠血清TNF-α、IL-6、IL-1β含量均有所降低，黑逍遥散高剂量组大鼠血清TNF-α、IL-6、IL-1β含量显著降低。结论：有效控制AD患者体内炎性因子表达是黑逍遥散的重要作用机制。