白细胞介素-1单核苷酸多态性与甘肃回族、东乡族人群慢性牙周炎的相关性研究

摘要

Objective To examine and analyze the intedeukin (IL) -lB gene single nucleotide polymorphism(SNP) at positions +3954, and explore the association between SNP and risk of chronic periodontitis (CP) among the Hui and Dongxiang minorities in Gansu province. Methods CP group consisted of 215 patients from Hui and Dongxiang and healthy control group consisted of 219 subjects. Anti-coagulated peripheral blood sample was obtained from each subject and genomic DNA was extracted from each sample. SNP at IL-lB+3954 were analyzed by standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Results Statistically significant differences were found in genotype and allele frequencies between CP group and healthy control group. The exist of position alleles C increased the incidence of CP. The trend of frequency distribution of gene polymorphism above in Dongxiang was the same as that of Hui. Conclusion The present study reveals that IL-1B+3954C/T genotypes are significantly associated with the risk of CP. These results indicate that genetic polymorphism is significantly associated with the risk of CP among the Hui and Dongxiang minorities in Gansu province.%目的 通过检测并分析白细胞介素(IL)-1B+3954位点的单核苷酸多态性(SNP),探索其与甘肃回族与东乡族人群慢性牙周炎的关系.方法 收集215例回族、东乡族慢性牙周炎患者及219例正常对照组健康者的静脉血,提取外周静脉血白细胞DNA,采用聚合酶链式反应-限制性片段长度多态性技术(PCR-RFLP)方法检测IL-1B+3954位点等位基因频率以及基因型频率.结果 牙周炎组与正常对照组间基因型频率分布、等位基因频率分布差异都有统计学意义,C等位基因的存在使患牙周炎的危险性增加.IL-1B+3954基因多态性在回族与东乡族患者中的分布规律相似.结论 本研究证明了IL-1B+3954C/T基因型与甘肃回族、东乡族慢性牙周炎的发病具有密切的相关性,显示遗传多态性与甘肃回族、东乡族慢性牙周炎的发病率有关.