目的 探讨白细胞介素12p40(IL-12p40)及干扰素-γ(IFN-γ)在口腔扁平苔藓(OLP)病损形成及发展中的意义.方法 正常口腔黏膜组织11例,OLP组织43例,采用免疫组织化学Envision二步法检测IL-12p40和IFN-γ的表达情况,分析其与OLP患者临床病理特征的关系.结果 1)OLP组IL-12p40与IFN-γ的阳性表达率均高于正常对照组,差异有统计学意义(P<0.05).2)在OLP组中,IL-12p40阳性表达率在IFN-γ阳性组高于IFN-γ阴性组,差异有统计学意义(X2=5.828,P=0.016),IFN-γ和IL-12p40表达之间存在正相关关系(r=0.357,P=0.019).3)IL-12p40的阳性表达率在病程短于6个月组高于病程长于6个月组组(X2=7.935,P=0.005);基底细胞液化程度高分组中的IFN-γ阳性表达率高于低分组(X2=9.070,P=0.011).结论 IL-12可能通过促进典型Th1型细胞因子IFN-γ的产生参与了OLP病损区的病理损伤过程,在OLP局部病损形成初始阶段起到关键作用.%Objective To investigate the significance of cytokine interleukin-12p40 (IL-12p40) and interferon-γ (IFN-γ) in tissues formation and development of human oral lichen planus (OLP).Methods The tissues of 11 cases of normal oral epithelium and 43 cases of OLP were investigated for the expression of IL-12p40 and IFN-γ proteins by using Envision two-step immunohistochemistry.The correlations between the expressions of these two cytokines,and their clinical and pathological significance in OLP were analyzed.Results 1)IL-12p40 and IFN-γ proteins were up-regulated in OLP comparing with that in normal oral mucosa and there was statistical significance between their difference(P＜0.05).2)The percentage of positive IL-l2p40 staining in OLP of IFN-γ positive group was higher than IFN-γ negative group and there was statistical significance between their difference(x2＝5.828, P＝0.016).A positive correlation was found between IL-12p40 and IFN-γ proteins in OLP(Spearman r＝0.357, P＝0.019).3)The percentage of positive IL-12p40 staining in OLP with short course (＜6 months) was higher than that in OLP with long course (≥6 months; x2＝7.935, P＝0.005), and a significant association was found between IFN-γ over expression and the degeneration of base cells in OLP lesions (x2＝9.070, P＝0.011).Conclusion These results indicate that at the primary phase of OLP, IL-12 may drive the pathological destruction in OLP lesions by elevating IFN-γ protein locally.IFN-γ may play an important role for the pathological destruction in OLP lesions.
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