Objective To detect the biological influence to human tongue squamous cell carcinoma (TSCC) cells of micro ribonucleic acid-21 (miR-21). Methods Referring to mature miR-21 sequence, the sense and antisense oligonucleotide (sense-miR-21 and AS-miR-21) modified by 2'0-Me were designed to transfect into TSCC cells (Tca8113 and high metastasis cells) by liposome transfection technology, in order to establish an in vitro TSCC cell model. The expression changes of miR-21 in the transfected cells were detected with real-time fluorescence quantitative polymerase chain reaction (real-time PCR). The changes of cell proliferation, cell cycle, cell early apoptosis, cell migration and invasion capabilities were detected respectively by the technologies of methyl thiazolyl tetrazolium CMTT), flow cytometry, Annexin V cell early apoptosis assay, scratch assay and Transwell asbay, to check AS-miR-21's effect on the biologicalcharacteristics of human TSCC cell lines. Results For the TSCC cells, the antisense oligonucleotide of targeting miR-21 could effectively inhibit cell proliferation, promoted cell apoptosis, and inhibited the capability of cell's migration and invasion. Conclusion The expressions of miR-21 decrease after AS-miR-21 transfected into TSCC cells, and miR-21 can affect biological behavior of TSCC cells.%目的 研究微小核糖核酸-21 (miR-21)对人舌鳞状细胞癌细胞系生物学特性的影响.方法 将2'O-Me修饰的正义和反义miR-21寡核苷酸序列通过脂质体转染技术转染到舌鳞状细胞癌细胞Tca8113及其高转移株内,采用实时荧光定量聚合酶链式反应检测转染后miR-21在细胞中的表达变化,通过甲基噻唑基四唑(MTT)法、流式细胞术、Annexin Ⅴ细胞早期凋亡检测、划痕实验及Transwell实验分别检测细胞增殖能力、细胞周期、细胞早期凋亡情况、细胞迁移及侵袭能力的变化.结果 靶向miR-21的反义寡核苷酸可以有效地抑制Tca8113及其高转移株细胞的增殖,促进细胞凋亡,并抑制细胞的迁移和侵袭能力.结论 细胞内转染反义miR-21寡核苷酸序列后,可以降低舌鳞状细胞癌细胞中miR-21的表达,并对舌鳞状细胞癌细胞的生物学行为产生影响.
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