Notch信号通路分子在舌鳞癌中的表达及意义

摘要

Objective To explore the expression of Notch signaling receptors Notch1,Notch3 and its ligand Jagged1,Jagged2 in tongue squamous cell carcinoma(TSCC).Methods mRNA and protein expression levels of tissue samples from 74 cases of tongue cancer patients and human tongue cell line Cal-27 were detected by reverse transcriptasepolymerase chain reaction (RT-PCR),immunohistochemistry and Western blot.Its relationship with cell proliferation and clinical pathology was analyzed.Results mRNA and protein expression were detected in tongue cancer tissues,adjacent tissues and cell lines.Notch1 and Notch3 protein expression in tongue cancer was higher than the adjacent tissues.Jagged1 and Jagged2 protein expression in tongue cancer and adjacent tissues had no difference.Notch1 and Notch3 protein had correlation with tongue cancer clinical staging.Pathway protein expression had no correlation with pathological grade,age,gender.Notch1 protein expression in lymph node metastasis-positive cases was higher than in lymph node metastasis-negative cases.The expression of Notch3 and Jagged2 had correlation.Jagged1 expression grade in metastasis-positive cases was higher than in negative cases.Conclusion Notch signaling molecules have active expression in TSCC and may play important roles in tongue cancer development.%目的 明确Notch信号通路受体Notch1、Notch3及其配体Jagged1、Jagged2在舌鳞癌中的表达及其意义.方法 通过逆转录聚合酶链反应、免疫组织化学和Western blot检测74例舌癌患者的组织标本(舌癌及癌旁组织)和人舌癌Cal-27细胞株中Notch1、Notch3、Jagged1和Jagged2 mRNA与蛋白质的表达水平,分析其与细胞增殖及临床病理的关系.结果 舌癌、癌旁组织及Cal-27细胞株中均检测到Notch1、Notch3、Jagged1、Jagged2 mRNA和蛋白质的表达.Notch1和Notch3蛋白在舌癌中的表达率高于癌旁组织,而Jagged1和Jagged2蛋白在舌癌和癌旁组织中的表达率无明显差异.Notch1和Notch3蛋白的表达和舌癌的临床分期具有相关性.4种信号蛋白的表达与患者的年龄、性别和临床病理分级无相关性.Notch3和Jagged2的表达具有相关性.Notch1蛋白在淋巴结转移阳性病例中的表达率高于转移阴性的病例,Jagged1在转移阳性病例的表达分级高于阴性病例.结论 Notch信号通路分子在舌鳞癌中表达活跃,与舌癌的发生发展有重要的相关性.