目的 探讨对体外培养的成骨细胞增殖活性促进作用最佳的17-β雌二醇的浓度及作用时间、流体剪切力(FSS)的力值及作用时间,以及此双因素共同作用对成骨细胞增殖活性的影响.方法 成骨细胞系MC3T3-E1细胞传代培养后,分别对其施加不同浓度的17-β雌二醇和不同力值的FSS,采用MTT法检测细胞的增殖情况,并检测ALP活性,筛选出最佳的浓度及力值；再将二者共同作用于MC3T3-E1细胞,检测其增殖情况和ALP活性.结果 浓度为10-8mol·L-1的17-β雌二醇作用5d时,MC3T3-E1细胞的增殖及ALP活性优于其他17-3雌二醇处理组；FSS力值为12×10-5 N作用60 min时细胞的增殖及ALP活性大于其他FSS处理组.当二者同时作用于MC3T3-E1细胞时,细胞的增殖活性大于任一单因素处理组.结论 17-β雌二醇和FSS对成骨细胞活性的促进作用皆有一个适宜的阈值；二者具有协同作用,对成骨细胞分化及增殖功能的影响优于单一因素作用.%Objective To explore the suitable level and action time of 17-β estradiol and fluid shear stress (FSS) and their combined effect on the proliferation of rat osteoblasts in vitro. Methods MC3T3-E1 osteoblasts were adopted after subcultured and different concentrations of 17-β estradiol and FSS values were applied respectively on MC3T3-El, the suitable level of 17-β estradiol and FSS were selected through MTT and alkaline phosphatase (ALP). Then the two factors at the suitable level were applied simultaneously to MC3T3-E1 to detect the proliferation activity. Results Seventeen-β estradiol (10-8 mol·L-1) for 5d and 12×l0-5 N FSS for 60 min exhibited better effects on the proliferation activity than the other groups respectively, and the combined effect of both factors was better than any single-factor treated group. Conclusion Both 17-β estradiol and FSS have a suitable threshold in promoting proliferation of osteoblasts, and two-factor treated group exhibits better effect than any other single-factor treated groups. Therefore 17-β estradiol and FSS have a synergetic action on differentiation and proliferation of osteoblasts.
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