目的：探讨单纯缺血再灌注引起的大鼠颌下腺损伤-应激反应及其对颌下腺分泌功能的影响。方法建立大鼠颌下腺原位缺血再灌注模型，以对侧腺体作为对照组，实验侧颌下腺经历90min缺血，分别再灌注1、12、24、72h后，检测各组腺体的分泌量，组织学变化，活性氧水平及细胞凋亡情况。结果大鼠颌下腺经历缺血再灌注后1h和12h，腺体分泌量显著降低，至再灌注72h腺体的分泌量逐渐恢复正常。组织学观察发现，经历缺血再灌注后，腺体组织开始出现水肿及炎症细胞浸润，至再灌注12h最为严重，其后逐渐恢复至正常。腺体组织活性氧水平及细胞凋亡也表现出相同的变化趋势，经历再灌注1h和12h后，腺体组织内活性氧信号增多，细胞凋亡水平显著增高。结论缺血再灌注损伤是移植腺体早期分泌功能低下的重要原因之一。%Objective To investigate the injury stress responses caused by ischemia reperfusion and its effects on the salivary secretory function of rat submandibular glands. Methods An in situ ischemia reperfusion experimental model of rat submandibular glands was developed. The rat submandibular glands were subjected to 90 min of ischemia without denervation followed by reperfusion for 1, 12, 24, and 72 h. Salivary secretion, histological changes, reactive oxygen species (ROS) levels, and cellular apoptosis of the involved submandibular glands were detected after reperfusion. Results The secretory function of the glands decreased at 1 and 12 h, and the saliva secretion gradually had the same value as that of the control sample 72 h after reperfusion. Increasing inflammatory cells infiltration, cellular atrophy, and tissue edema were observed especially after reperfusion for 12 h. The level of ROS and the number of apoptotic cells exhibited the same tendency, and higher ROS levels and more apoptosis cells 1 and 12 h after reperfusion were observed. Conclusion Our study suggests that ischemia reperfusion can cause a series of injury stress responses in submandibular glands, which might have an important function in the early phase dysfunction of transplanted submandibular glands.
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