In order to determinate the alkaloids (nicotine, nornicotine, myosmine, anabasine and anatabine) in total particulate matter ( TPM) of mainstream cigarette smoke puff by puff, cigarettes were smoked by a modified Borgwaldt RM20/CS smoking machine.TPM per puff of 20 cigarettes was trapped on individual Cambridge filter pad, the filter pad was extracted under ultrasonic, then centrifugalized, the supernatant liquid of the extract was analyzed by GC/MS.The results showed that; 1) the relative standard deviations (RSDs) of the 5 alkaloids ranged from 1.33％ to 7.58％ with the recoveries from 94％ to 106％ ; 2) the per puff deliveries of TPM, nicotine and myosmine in mainstream cigarette smoke showed an ascending trend, especially for TPM and nicotine; 3 ) the delivery of nornicotine in mainstream smoke of all samples was lower, there was no obvious change between puffs, and the total content of nornicotine in mainstream smoke of blended type cigarette sample F was the highest.4) the per puff deliveries of the 5 alkaloids in mainstream smoke of low tar cigarettes and blended cigarettes, samples D, F and G, were lower than those of other cigarettes.This method is suitable for determining alkaloids in TPM of mainstream cigarette smoke.%为测定卷烟烟气总粒相物中5种生物碱(烟碱、降烟碱、麦斯明、假木贼碱和新烟草碱),采用经改造的吸烟机对卷烟进行抽吸,用剑桥滤片捕集20支卷烟的每口烟气总粒相物,滤片经超声波萃取、离心后,取上层清液用GC/MS进行测定分析.结果显示:①利用该方法在所测定的5种生物碱中,测定结果的相对标准偏差为1.33%～7.58%,各生物碱的加标回收率为94%～106%;②逐口TPM量、逐口烟碱量以及逐口麦斯明量逐渐升高,逐口TPM量和逐口烟碱量在每口间传递的规律性最强;③降烟碱量都比较低,没有发现降烟碱逐口量随口数的明显变化趋势,每口之间都比较平稳,混合型卷烟F的降烟碱总量最大;④卷烟D、F和G等低焦油或混合型卷烟的主流烟气TPM中5种生物碱单口传递量较之于其它卷烟偏低.该方法适用于卷烟主流烟气总粒相物中生物碱含量的测定.
展开▼
