m型硫氧还蛋白对马铃薯Y病毒侵染烟草的影响

摘要

In order to further confirm the interactive relationships between virus and its host, a gene featured the characters of m-type thioredoxin in tobacco cell was cloned by homologous alignment and named as NtTrm. The expression of NtTrm and its relationship with PVY accumulation were studied by protoplast transient transformation, virus-induced gene silencing (VIGS), semi-quantitative RT-PCR and Northern blot test technologies. The results showed that the expression of NtTrm in leaves of cv. Honghuadajinyuan was obviously up-regulated 10 days after PVY inoculation; the down-regulation of NtTrm significantly promoted the accumulation of PVY in leaves of Nicotiana benthamiana; while the over-expression of NtTrm in tobacco protoplast obviously inhibited the accumulation of PVY viral RNA. It preliminarily indicated that NTtrm could be induced by PVY infection and its up-regulated expression might affect the PVY infection in tobacco cells.%为进一步明确病毒与寄主间的相互作用关系,通过同源比对在烟草细胞中克隆到具有m型硫氧还蛋白(m-type thioredoxin)特征的基因,暂命名为NtTrm.利用原生质体瞬时转化技术、病毒诱导的基因沉默技术(VIGS)、半定量-PCR 以及Northern blot检测技术研究NtTrm的表达以及NtTrm表达与PVY积累的关系.结果表明,在PVY接种10 d后红花大金元叶片中NtTrm表达明显上调;在本氏烟草叶片中NtTrm下调显著促进了PVY在烟草叶片中的积累;而在烟草原生质体中NtTrm过量表达则会明显抑制病毒RNA的积累.初步说明烟草细胞内编码m型硫氧还蛋白的基因受PVY侵染而被诱导表达,这类基因的上调表达可能影响PVY在烟草细胞中的侵染.