Suitable soil microbial environment is a necessary condition for quality tobacco production. In tobacco fields, land consolidation can stir soil layer in a large scale which in turn influences soil microbial community. To study the effects of combining traditional fertilizers with biological organic manure and/or phosphorus fertilizer on the bacterial community in tobacco fields of yellow soil type after land consolidation, experiments were conducted in selected fields for three consecutive years to evaluate the quality of tobacco leaves. The results showed that combining traditional fertilizers with biological organic manure and/or phosphorus fertilizer improved the structure and diversity of bacterial community and increased the number of bacterial species. The relative abundances of Chloroflexi, Nitrospira, Proteobacteria and 51.4% of bacterial genera were higher than those of traditional fertilizer treatment alone. The improvement of the bacterial community structure in tobacco fields significantly increased the yield and output value of flue-cured tobacco and the percentage of high and middle grade leaves. Therefore, biological organic manure (applied to the soil before seedling transplanting at a rate of 675 kg/ha) and rhizosphere soil at 1 500 kg/ha (the mixture of 1 372.5 kg ash soil, 75 kg biological organic manure, 15 kg compound fertilizer and 37.5 kg phosphate fertilizer) were recommended for the tobacco fields of yellow soil type immediately after land consolidation.%适宜的土壤微生物环境是烟草优质适产的必要条件，而烟田整理后土层被大规模搅动，对土壤微生物群落组成产生影响。为明确生物有机肥与磷肥配施对新整理黄壤烟田土壤细菌群落结构和烟叶产质量的影响。以新整理黄壤烟田为对象，连续3年进行了定点田间试验，结果表明，生物有机肥和磷肥配施可以改善新整理黄壤烟田土壤细菌群落结构，烟田土壤细菌群落多样性提高，菌类数增加，绿弯菌门、硝化螺旋菌门、变形菌门及51.4%的菌属相对丰度均高于传统施肥，尤其是生物有机肥和磷肥同时配施效果更佳；烟田土壤细菌群落结构的改善使烤烟的产量、产值与上中等烟比例显著提高。因此，对于新整理黄壤烟田推荐基施生物有机肥675 kg/hm2配合基施根际营养土1500 kg/hm2（1372.5 kg火土灰、75 kg生物有机肥、15 kg复合肥与37.5 kg磷肥均匀混合）的施肥方式。
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