Objective: To study the effect of dual dibutyryl cyclic AMP(dbcAMP) on the proliferation of SW579 cell line. Methods : The growth activity of cells were detected by MTT, after SW579 cells were treated with dbcAMP (0.5, 1.0, 2.0 mmol/L) for 24 h. At the level of 0.5 mmol/L, the activity of maturation promoting factor (MPF) were tested through ELISA. The protein expression of the phosphorylation level of cdc2-Tyrl5 was measured by Western blot. Changes of the cell cycle were detected by flow cytometry. Results: Compared with control group, the growth activity of SW579 cells was reduced after treatment with different levels of dbcAMP. Results of ELISA showed that the activity of MPF was (22.87±2.57) ng/L after treatment with 0.5 mmol/L dbcAMP for 24 h, which was significantly lower than that of control group (115.5±7.53) g/L it = 3.324,P < 0.05). The protein express of the phosphorylation level of cdc2-Tyrl5 was higher in test group (0.258 ±0.026) than that of control group (0.142±0.009, t = 7.424,P < 0.05). Compared with control group, the number of SW579 cells had no significant difference in GO/ G1 phase, reduced in the S phase and increased obviously in the G2/M phase after treating with 0.5 mmol/L dbcAMP. Conclusion: dbcAMP could significantly reduce the vitality of SW579 cells, by which the cell proliferation was significantly inhibited.%目的:探讨双丁酰环腺苷酸(dbcAMP)对甲状腺鳞癌SW579细胞株增殖的影响.方法:将dbcAMP 0.5、1.0、2.0 mmol/L作用于甲状腺鳞癌SW579细胞24 h后,用四甲基偶氮唑蓝比色(MTT)法检测SW579细胞增殖的变化；经dbcAMP 0.5 mmol/L作用24h后,ELISA法检测细胞成熟促进因子(MPF)的活性,Western Blot方法检测cdc2-Tyr15磷酸化水平,流式细胞术检测细胞周期变化情况.结果:与对照组相比,经不同浓度dbcAMP处理的SW579细胞株的增殖均受抑制.在0.5 mmol/L dbcAMP作用下24 h,SW579细胞MPF活性为(22.87±2.57) ng/L,远小于对照组的(115.50±7.53) ng/L,差异有统计学意义(t=3.324,P＜0.05).实验组cdc2-pTyr15磷酸化水平(0.258 ±0.026)高于对照组的(0.142±0.009),差异有统计学意义(t=7.424,P＜0.05).与对照组相比,应用0.5 mmol/L dbcAMP处理细胞之后,G0/G1期无显著变化,S期细胞数量减少,G2/M显著增多.结论:dbcAMP能够显著降低甲状腺鳞癌SW579细胞株的活力,使细胞增殖受到明显抑制.
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