浙贝母叶与浙贝母花醇提物的止咳、化痰及平喘活性研究

摘要

Objective To observe the effects of the ethanol extract of thunberg fritillary leaf (EETFL) and the ethanol extract of thunberg fritillary flower (EETFF) on relieving cough, sputum elimination and relieving asthma. Methods The cough relieving effects of EETFL and EETFF were studied in mouse cough model caused by ammonia water and in guinea pig cough model caused by citric acid. The sputum elimination effects of EETFL and EETFF were researched by the observation of tracheal phenol red shedding in mice. The asthma relieving effects were tested by spraying method in guinea pigs. Results EETFL can obviously inhibit the incubation period and cough frequency of the model mice and guinea pigs induced by ammonia water and citric acid (P<0.05), and significantly improve the tracheal phenol red excretion volume in mice (P<0.05), and obviously prolong the incubation period of asthma (P<0.05). EETFF can obviously inhibit the incubation period and cough frequency of the model mice and guinea pigs induced by ammonia water and citric acid (P<0.05), and significantly improve the tracheal phenol red excretion volume in mice (P<0.05), but EETFF couldn’t prolong the incubation period of asthma evidently. Conclusion EETFL has obvious activity of relieving cough, eliminating phlegm and relieving asthma. EETFF has obvious activity of relieving cough and eliminating phlegm, but EETFF has no anti-asthmatic activity under the current dose.%目的：考察浙贝母叶与浙贝母花醇提物的止咳、化痰及平喘活性。方法分别采用小鼠氨水引咳法和豚鼠枸橼酸引咳法研究浙贝母叶与浙贝母花醇提物的止咳活性；小鼠气管酚红排泌法研究化痰活性；豚鼠整体动物引喘法研究平喘活性。结果浙贝母叶醇提物可明显延长小鼠和豚鼠的咳嗽潜伏期并减少咳嗽次数，显著增加小鼠呼吸道黏膜酚红的排泌量，显著延长豚鼠哮喘潜伏期（均P＜0.05）；浙贝母花醇提物可明显延长小鼠和豚鼠的咳嗽潜伏期并减少咳嗽次数，显著增加小鼠呼吸道黏膜酚红的排泌量（均P＜0.05），但不能延长豚鼠哮喘潜伏期（P＞0.05）。结论浙贝母叶醇提物具有明显的止咳、化痰及平喘活性；浙贝母花醇提物具有明显的止咳、化痰活性，但现有剂量下，没有平喘活性。