云南耿马蔗区甘蔗宿根矮化病的调查及病原检测

摘要

Ratoon stunting disease （RSD） which cause tremendous damage to sugarcane production is an important sugarcane disease around the world. The key to control epidemic of the RSD and to promote disease- free seedings treated with warm water is to investigate the occurrence and distribution of RSD. In this study, 97 sugarcane samples were collected from fields in Gengma county of Yunnan province and then tested by PCR and I-ELISA. The results showed that of the tested 97 samples, 73 samples （75.26%） were RSD positive, confirming the presence of RSD in Gengma. More systematic analysis of RSD occurrence in different cultivars of different seasons and regions provided important information for controlling RSD effectively and amplifying size of disease-free seedings in Gengma.%甘蔗宿根矮化病（Ratoon Stunting Disease，RSD）是一种世界性的重要甘蔗病害，对甘蔗生产危害极大。摸清蔗区RSD的分布、发生为害情况，是科学推广温水脱毒健康种苗，有效防控甘蔗宿根矮化病的关键。本文对云南耿马蔗区甘蔗宿根矮化病的发生和分布进行了调查和田间采样，采用PCR和I-ELISA法，对田间采集的97个样本进行RSD检测。结果表明：73个样本为阳性，阳性检出率75．26％，24个样本为阴性，确认耿马蔗区存在RSD；通过系统分析，明确了不同品种、不同植期、不同蔗地RSD发生状况，为耿马蔗区推广应用温水脱毒健康种苗、有效防控甘蔗宿根矮化病提供了科学依据。