以蓝莓良种‘灿烂’半木质化茎段为材料，通过对增殖培养基以及生根培养基的筛选，建立蓝莓高效组培快繁技术体系。结果表明，最佳的增殖培养基为1/2MS＋2.0 mg·L-1 ZT＋0.01 mg·L-1 NAA＋30 g·L-1白糖＋6.5 g·L-1琼脂，芽诱导率为87%，增殖系数达3.6；ZT 1.0～2.0 mg·L-1对不定芽诱导效果明显，随着ZT浓度的提高，愈伤组织产生越明显，影响增殖苗的质量。最佳生根培养基为1/2MS＋1.5 mg·L-1 IBA＋0.01 mg·L-1 NAA ＋25 g·L-1白糖＋7.0 g·L-1琼脂，pH值5.8，生根率70%，IBA浓度从0.5 mg·L-1提高到1.5 mg·L-1，生根越多，根系越壮，但也产生更多愈伤组织影响生根质量。炼苗10 d后移栽至草炭土，成活率78%。%In order to obtain efficient tissue cultural propagation technology of blueberry, the semi-lignification shoots of blueberry (Britewell) used as explants, propagation medium, rooting culture medium was selected. The result showed that 1/2MS+2.0 mg·L-1 ZT+0.01 mg·L-1 NAA+30 g·L-1 sugar+6.5 g·L-1 agar+pH 5.8 is the optimal propagation medium to blueberry (Britewell), has 87%rate of buds induction on aseptic seeding, and the coefficient multiplication is 3.6,Improved ZT concentration, more callus emerged in propagation medium. its contributed to improving multiplication but also caused more thin aseptic seeding; 1/2 MS+1.5 mg·L-1 IBA+0.01 mg·L-1 NAA+25 g·L-1 sugar+7 g·L-1 agar+pH 5.8 is the optimal rooting medium, has 70% rooting percentage. Being acclimatized for 10 d, the rooted plantlets were transplanted to turfy-soil and the survival rate was 78%. Control callus produced and cheaper hormones applicated is the key of efficient tissue cultural propagation technology of blueberry.
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