实验性脑出血后大鼠行为学和血肿周围组织病理学的研究

摘要

目的 观察大鼠脑出血后不同时间点神经行为学和血肿周围脑组织病理学的特点.方法 将成年SD大鼠随机分为假手术组和脑出血组;脑出血组大鼠通过立体定向术向脑内注入VII型胶原酶制成脑尾状核出血模型,并按不同时间点(1、3、7、14、28 d)分为5个亚组.采用神经功能评分和HE染色分别观察脑出血大鼠神经行为学和脑组织形态学的改变.结果 与假手术组相比,脑出血组大鼠的神经行为学评分3 d时最明显[3 d与1、7 d无明显差异(P＞0.05);与14 d和28 d有显著差异(P＜0.05)].脑出血后1 d在尾壳核区域可见血肿形成,呈椭圆形或不规则形;3d时脑水肿明显;7 d时血肿周围脑组织有胶质细胞增生;14 d时血肿区逐渐形成不规则囊腔;28 d时囊腔仍然存在,出血周边区见胶质细胞进一步增多.结论 脑出血后神经行为学的改变、血肿及囊腔的形成与出血时间有关.%Objective To observe the characteristics of neurological behavior and pathology of perihematoma region of rats following intracerebral hemorrhage. Methods The adult SD rats were randomly assigned into two groups: sham group and intracerebral hemorhage group. The rats of intracerebral hemorrhage group were dealed by stereotaxic method and injected Type Ⅶ collagen enzyme was injected into caudate nuclear of rats and they were subdivided into 5 subgroups according to various time(l d,3 d,7 d,14 d and 28 d). Neurological scores and HE Staining were used to investigate changes of neurological behavior and pathology of perihematoma region of rats following intracerebral hemorrhage. Results Compared with sham group, behavioral scores in rats of intracerebral hemorhage group were most obviously in 3 d(P＞0.05 on 1 d,3 d,7 d; P＜0.05on 14 d and 28 d). Ovalshap or irregular hematoma were seen in caudate nuclears at 1 day following intracerebral hemorrhage in rats : Cerebral edema was obviously found : there was gliocytes proliferate in the border are as at 7 days; Irregular capsular space of hemorrhagic focus began to form at 14 days. The gliocyte increased in the border areas and the capsular space still remained. Conclusions The change of neurological behavior, the absorption of hematoma and form of capsular space are related to hemorrhagic time.