HPLC法测定胆仙胶囊中绿原酸的含量

摘要

目的：建立测定胆仙胶囊中绿原酸含量的方法。方法采用高效液相色谱法。色谱柱为依利特C18（4．6mm ×250mm，5μm），流动相为乙腈－0．1％磷酸水溶液（20∶80，V／V），流速为1．0mL・ min －1，检测波长为327nm，进样量为10μL。结果绿原酸的质量浓度线性范围为14．0～140μg・ mL－1（r＝0．9992，n＝6）；平均加样回收率为99．52％，RSD为0．73％（n＝9）。结论本方法快速、简便、准确、重现性良好，可用于该制剂的质量控制。%OBJECTIVE To establish a method for determination of chlorogenic acid in Danxian cap-sules.METHODS HPLC method was adopted.The column was elite C18 (4.6mm ×250mm,5μm) .The mobile phase was acetonitrile-0.1%phosphoric acid solution (20∶80,V/V),the flow rate was 1.0mL・ min -1 and detec-tion wavelength was 327nm,the injection volume was 10μL.RESULTS Chlorogenic acid concentration linear range of 14.0~140μg・ mL-1 ( r =0.9992 , n =6 );the average recovery was 99.52% with RSD of 0.73%( n =9 ) .CONCLUSION The method is rapid,simple,accurate,reproducible,and can be used for the quality control of this preparation.