HPLC法测定维生素C注射液的含量

摘要

OBJECTIVE To establish a method for the determination of vitamin C injection by HPLC.METHODS Scientific HYPERSIL ODS C18 Thermo (4.6mm ×250mm,5μm) chromatography column was used,mobile phase:A phase was 0.1% phosphoric acid water ,B phase was methanol;flow rate was 1mL・ min -1;detection wavelength was 245 nm;column temperature was 25℃.RESULTS The linear regression equation was A=3879545 X+9213 , R2 =0.9999 , the linear range was 0.0618 ~0.5562μg, the average recovery was 99.38%(RSD was 0.47%).CONCLUSION The method is simple,rapid,accurate and reproducible,and can be used for the determination of vitamin C injection .%目的：建立用HPLC测定维生素C注射液含量的方法。方法采用 Thermo Scientific HYPERSIL ODS C18（250mm ×4．6mm，5μm）色谱柱，流动相：A相：0．1％磷酸－水，B相：甲醇；流速1mL・ min －1；检测波长245nm；柱温25℃。结果线性回归方程为A＝3879545X＋9213，R2＝0．9999，线性范围0．0618～0．5562μg，平均回收率为99．38％（ RSD 0．47％）。结论该方法操作简单，快速准确且重复性好，可用于维生素 C注射液的含量测定。