Objective To verify the feasibility and accuracy of the microsphere diffusion method by the method of electron microscopy; meanwhile, to measure and compare the four different micro-gaps of the four different implant-abutments stressed by different horizontal loads. Method Four kinds of implant based micro-gap models were constructed in vitro ( Group A: Anatomic all ceramic abutment; Group B: Syn Octa octagonal titanium base; Group C: Procera titanium base all ceramic abutment; Group D: Procera all ceramic abutment, Polystyrene microspheres were added into the implants ( 0.2-5.0 μm). At the same time, the different horizontal loads ( 0, 50, 100, 150 N) were given. The maximum particle size of micro diffusion was measured by laser particle size analyzer. At the same time, the time required to measure the maximum width was recorded. On the other hand, scanning electron microscopy was used to measure the width of the slit of the 8 observation points of each slit model, and the maximum value was obtained. The differences between the two methods were analyzed by t test. Results (1) There was no significant difference in the accuracy of the micro slit width between the microspheres diffusion method and the electron microscope ( P<0.05). (2) Micro diffusion method to detect micro slit width took up to 15 minutes. (3) When there was no external load, the micor-gaps of A and D groups were significantly larger than B, C groups ( P<0.05). (4) In B, C groups, 100 N and 150 N load micro-gaps were greater than 0 N ( P<0.05) ; 150 N load of micro-gap was greater than 50 N ( P<0.05). Conclusion Microsphere diffusion method is simple, efficient and accurate to measure the maximum width of micro-gap between implant and abutment. The micro-gap of the implant-procelain interface is significantly larger than that of the implant-titanium interface, meanwhile, the micro-gap of the implant-titanium interface will increase significantly when stressed by the larger horizontal loads.%目的 利用电镜测量法验证微球扩散法的可行性和精确性;同时使用该法测量并比较4种不同种植体-基台在不同水平载荷作用下微缝隙的大小.方法 离体构建4种种植体-基台微缝隙模型 (A组:Anatomic全瓷基台; B组:Syn Octa内八角钛基台; C组:Procera钛基底全瓷基台; D组:Procera全瓷基台) , 在种植体内放入聚苯乙烯微球乳液 (粒径0.2～5.0μm) , 同时给各组基台以不同的水平向载荷 (0、50、100、150 N) , 使用激光粒度仪检测扩散通过微缝隙的最大微球直径, 记为微缝隙的最大宽度.同时记录测得该最大宽度所需的扩散时间.另一方面, 使用扫描电镜测量每个模型的8个观测点的微缝隙宽度, 取最大值.比较两种检测方法的差异.结果 (1) 微球扩散法和电镜测量法对于微缝隙宽度的检测精度无统计学差异 (P<0.05) ; (2) 微球扩散法检测微缝隙宽度最多耗时15 min; (3) 无外力载荷时, A、D组微缝隙显著大于B、C组 (P<0.05) ; (4) B、C组, 100 N和150 N载荷的微缝隙大于0 N (P<0.05) ; 150 N载荷的微缝隙大于50 N (P<0.05) .结论 微球扩散法可简便、高效、准确地测量种植体-基台间微缝隙的最大宽度.无载荷条件下, 种植体-全瓷界面的微缝隙显著大于种植体-钛界面的微缝隙;在较大的水平载荷作用下, 种植体-钛界面微缝隙会显著增大.
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