无牙颌患者口腔菌群组成的焦磷酸测序分析

摘要

目的 研究无牙颌患者的口腔菌群组成.方法 收集11例无牙颌患者口底/舌腹、舌背、唇颊前庭、硬腭/牙槽嵴顶、唾液中的菌斑样本,构建细菌16S rRNA基因文库,采用焦磷酸测序法对16S rRNA基因的V2-V3可变区进行序列分析,完成无牙颌患者口腔菌群的物种鉴定.结果 11例无牙颌患者共测得51091条16S rRNA 基因序列,主要分布于厚壁菌门、变形菌门、拟杆菌门、放线菌门,多为链球菌属、奈瑟菌属、卟啉单胞菌属、罗氏菌属等,优势菌种为缓症链球菌、毗邻颗粒链菌、殊异韦荣菌、香茅醇假单胞菌、副猪嗜血杆菌、动物口腔奈瑟氏球菌、卟啉单胞菌、黏液罗氏菌.结论 牙列缺失对无牙颌患者的口腔菌群组成有一定影响.%Objective To explore the compositions of oral flora from edentulous individuals. Methods Bacteria plaque samples were collected from mouth floor/tongue abdomen,tongue back,labial/buccal vestibule,hard palate/alveolar ridge crest and saliva of 11 edentulous individuals.The microbial 16S rRNA gene clone libraries were then constructed.Using pryosequencing,sequencing anal-ysis of the V2-V3 variable zone of 16S rRNA was performed to execute species identification of oral flora from 11 edentulous individu-als. Results A total of 51 091 sequences, which were classified into Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria phyla,were identified by pyrosequening in the 11 edentulous subjects. Predominant genera included Streptococcus, Neisseria, Porphy-romonas,Rothia, etc. Streptococcus mitis, Granulicatella adiacens, Veillonella dispar, Pseudomonas citronellolis, Haemophilus pitt-maniae,Neisseria zoodegmatis,Porphyromonas sp and Rothia mucilaginosa were dominant species. Conclusion Complete absence of dentitions has an impact on the oral flora compositions of edentulous individuals.