Near infrared spectroscopy (NIRs) technology was utilized for assessing effects in treating traumatic brain edema (TBE). Firstly, models for rats with traumatic brain edema were copied according to Feeney's apparatus. Then rats were given mannitol with different dosages (large and little) according to their groups. Simultaneously rat's brain tissues were monitored in vivo and real-time by NIRs mini-invasive detector developed by the authors' laboratory. And the water content of the brain tis-sues was measured by the wet and dry weight method at 1, 6, 24, 72 and 120 h after the injury and the treatment. Then, effects in treating TBE with different dosages were assessed by analyzing reduced scattering coefficient (μ'_s) data measured by NIRs and brain water content (BWC)before and after injecting dehydration. Finally, the authors found that reduced scattering coefficient (μ'_s) of rat's local cortex is a good indicator of assessing effects of treatment of TBE and that may be a preferable approach to assessing effects in vivo and real-time in treatment of brain injury.%通过近红外光谱技术对创伤性脑水肿治疗效果进行了评估.首先采用Feeney's自由落体撞击法建立SD大鼠创伤性脑水肿模型.然后利用两种不同剂最的苷露醇进行脱水治疗,同时应用自制的近红外(near infrared spectroscopy:NIRs)微创探头对腑组织进行在体实时监测,作为对比同时采用干湿比重法测量各监测时间点的脑水含量.最后根据近红外光谱技术实时监测的数据分析给药前后脑组织皮质优化散射系数(Reduced scattering coefficient:μ'_s)的变化规律,并结合脑水含量的变化情况,评估了不同剂量的甘露醇治疗创伤性脑水肿的疗效.根据实验结果得到结论:大脑皮层局部优化散射系数可以作为苷露醇疗效的实时评估参考指标参数,并且这种方法可以在体实时监测而不需处死动物,为将来颅脑损伤的实时疗效评估提出了一种方法.
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