四苯硼钠对罗丹明B具有荧光猝灭作用,使荧光信号强度减弱甚至消失,而盐酸西替利嗪对罗丹明B-四苯硼钠体系具有反猝灭作用,又能使该体系荧光信号增强,且荧光信号增强程度与药物浓度成线性关系.该文以491 nm为激发波长,以610 nm作发射波长,测量空白液与试液荧光强度之差△F=F1-F0,根据△F值与溶液中的盐酸西替利嗪浓度呈正比关系,建立了一种反荧光猝灭测定盐酸西替利嗪新方法,该方法线性回归方程为△F=0.727 5cm-2.357,相关系数R为0.997 2,线性范围3.50～129.3 mg·L-1检出限为1.05 mg·L-1,方法RSD为1.2%.用本方法对不同厂家生产的盐酸西替利嗪片以及盐酸西替利嗪胶囊样品进行测定,测定值与药品标示量基本相符,加标回收率在92%～106%之间.%The sodium tetraphenylboron has the fluorescence quenching effect on rhodamine B, making the fluorescence signal intensity weaken or even disappear. But cetirizine dihydrochloride has the anti-quenching effect on rhodamine B-the fluorescence signal is enhanced, and there was a linear relationship between the fluorescence signal enhancement degree and drug concentrationt. In the present paper, 491 and 610 nm were chosen as the excitation and emission wavelength for measurement of the fluorescence intensity difference △F= Fl —Fo of blank solution and test solution, and according to positive correlation between the value of △F and cetirizine hydrochloride concentration of test solution, a new method of anti-fluorescence quenching for the determination of cetirizine hydrochloride was established. The linear regression equation was △F = 0. 727 Sm—2. 357, correlation coefficient was 0. 997 2, linear range was 3. 50～129. 3 mg · L-1 , the detection limit was 1.05 rng · L-1 and RSD was 1.2％. The cetirizine hydrochloride tablets and cetirizine hydrochloride capsules from different manufacturers were determined by this method, the measured values were basically in line with the labeled amount of drugs, and the recovery rate was between 92％ and 106％.
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