油橄榄叶中总黄酮含量测定方法探讨

摘要

A quantitative determination method of the total flavonoids in Olea europaea leaves using rutin as a standard substance was developed. Two coloration systems including Al(NO3 )3-NaNOs-NaOH and AlCl3 were both used. The latter was selected as the optimal determination method by comparing with the results. The main interference of the matrix was discussed.The result showed that the AlCl3 coloration method is simple, accurate and reproducible with good linear relationship, and it can be looked as a reference method for the determination of total flavonoids in herbal materials or natural products.%建立以芦丁为对照品的油橄榄叶中总黄酮含量的测定方法.采用Al(NO3)3-NaNO2-NaOH和AlCl3两种显色法测定油橄榄叶中总黄酮的含量,并分别考察了两种方法对测定结果的影响.结果表明,AlCl3显色法适宜于油橄榄叶总黄酮的含量测定.该法操作简便,线性关系良好,准确度、重复性和稳定性较好,可用于油橄榄叶总黄酮含量测定,并为中药和天然产物中总黄酮的含最测定提供参考.