Extraction Process and Content Determination of Total Flavonoids in Ginkgo( Ginkgo biloba L. ) Leaves

摘要

[Objectives]This study was conducted to study the extraction process and the content determination of flavonoids in ginkgo(Ginkgo biloba L.)leaves.[Methods]Ethanol extraction and methanol extraction of total flavonoids in ginkgo leaves were studied,and the optimal extraction conditions for flavonoids were determined by orthogonal test;and with quercetin as reference substance,total flavonoid content in ginkgo leaves was determined by UV spectrophotometry.[Results]The optimal extraction process was 4 h of Soxhlet extraction with methanol;and the total flavonoid contents had a good linear relation in the range of 0.006 5-0.039 mg/ml(R 2=0.999 9),the average content was stabilized at 1.135%,and the average recovery of the method was 102.0%.[Conclusions]This study selected the optimal extraction process for total flavonoids in ginkgo leaves.The test method is simple with high accuracy and precision,and is suitable for the extraction and determination of total flavonoids in ginkgo leaves.