新型光动力学疗法光敏剂5-ALA-GNPs的制备及其光谱分析

摘要

通过聚二烯丙基二甲基胺盐酸盐和氯金酸制备阳离子纳米金,将纳米金和5-氨基乙酰丙酸(5-aminolevulinie acid,5-ALA)通过静电吸附作用有效结合得到新型光敏剂.应用共振瑞利散射光谱,紫外-可见吸收光谱,透射电镜和激光散射等方法对其进行了表征.结果表明通过这种方法纳米金与5-ALA可以有效结合.这种新型光敏剂对提高光动力学疗法临床疗效具有重要指导意义.%A novel strategy by means of poly diallyl dimethylammonium chloride (PDDA) was used to make highly mono-dispersed positively charged gold nanoparticles, and 5-ALA was immobilized onto the gold nanoparticles through electrostatic interaction. The conjugation of 5-aminolevulinic acid (5-ALA) and gold nanopartilces were characterized by the integrated tools of resonance Rayleigh scattering (RRS), UV-Vis absorption spectra, transmission electron microscopy (TEM) and laser light scattering. The results demonstrated that 5-ALA can be attached onto positively charged gold nanoparticles. This new photosensiti-zer is significant for enhancing PDT efficacy clinically.