医学美容和化妆品行业的进步与日俱进,为了揭示皮肤组织的细节,三维医学成像是必须的.为了减少侵入性,在本研究中应用“读出,而非写入”的想法,采用非电离辐射的光源与组织采样点的反射光谱实现皮肤的非侵入性成像,这种新技术称为“光谱分类成像术”.采用宽带光源与光谱仪收集扫描区域中各采样点的光谱曲线,根据其交叉相关系数分为几种类型的组织；使用相应于每一像素之组织类型的颜色填满每一个像素,可获得一幅彩色的组织断层影像.其次,探讨了光谱分类成像术的横向/纵向分辨率和穿透深度、展示了紫水晶样品与孔雀鱼样本的穿透造影结果、并探讨交叉相关系数大小与光源波长的数量等变因对于成像结果的影响.将宽带光源和光谱仪分别更换为RGB LED和微型光谱仪以取得样本光谱,该系统有潜力最小化为手持式医疗成像产品.与传统的医学成像技术相较,没有过于强烈的光源或有害人体的荧光染料被使用,将可减少非环保化妆品的过度使用,并促进医学美容产业的进展.%As skin is the exterior organ of human body,cosmetic industry advances year by year.To reveal the details of skin tissue,three-dimensional medical imaging is required.Based on the idea of "readout instead of write",a new scheme named spectral classification imaging (SCI) is proposed in the present study to reduce the invasiveness by applying the reflection spectra of the sample points for three-dimensional medical imaging.Broad-band light source and the spectrometer were employed to collect the spectra curves of scanned region,which were classified into several tissue types by their cross-correlations.A colorful tissue tomography can finally be obtained by filling in each image pixel the color indicating the corresponding tissue type.The lateral/longitudinal resolutions and penetration depth were analyzed to characterize the SCI system.The lateral resolution is based on the source's diffraction limit,the longitudinal resolution is by its depth-of-focus,and the penetration depth is equivalent to its skin depth.The imaging results of an amethyst of 0.6 mm (x-direction) ×0.6 mm (y-direction) with a total of 120×120 pixels per frame and a guppy fish of 3.2 mm (x-direction) × 2.4 mm (y-direction) of 160× 120 pixels,are presented to show the image quality.The effects of the cross-correlation coefficient and the number of source wavelengths on the imaging results were explored.The value of cross-correlation threshold determines the required time for imaging,the resulted number of tissue groups,and the variety of tissue colors in the imaging result.Owing to its virtual noninvasiveness and easy configuration,the SCI system is highly promising for practical uses.RGB LEDs possess merits of broad bandwidth,low cost,long lifetime,small volume,and are ready to be integrated into a multi-color source module.Replacing the wide-band light source and the spectrometer module with a composite RGB LED with discrete wavelengths and a micro-spectrometer for spectra retrieval,the system has great potential to be minimized as a hand-held product for noninvasive medical imaging.It leads to reduced use of non-eco-friendly cosmetics and extended advance of cosmetic dermatology.
展开▼
