四川紫金牛属药用植物的黄酮类活性组分的含量分析

摘要

用高效液相色谱法分析四川紫金牛属7种2变种药用植物的黄酮类活性组分,拟比较种与种间、同种不同入药部位之间的杨梅素、山奈酚和槲皮素的含量差异,探索紫金牛属植物的亲缘关系与药用组分含量的相关性,为紫金牛属不同药材质量控制标准的建立提供实验依据.色谱柱为Symmetry C18(4.6 mm×250 mm,5μm);流动相为甲醇:0.04％的磷酸溶液(50:50);体积流量:0.8mL/min;检测波长:368nm;柱温:30℃;进样量10μL.杨梅素、槲皮素、山奈酚获得良好基线分离,线性回归方程分别为Y=36 231 X-133 956,r=0.999 6;Y=45 758X-53 050,r=0.9995;Y=27 569X-7 263.5,r=0.9996;线性范围分别为0.064μg～1.920μg,0.084μg～2.522μg,0.061 μg～1.841μg;回收率分别为98.19％、97.88％、99.58％;RSD均小于1.6％;四川紫金牛属7种2变种药用植物均含有杨梅素、山奈酚、槲皮素.在相同条件下圆齿组植物的槲皮素含量均高于锯齿组植物;同种不同入药部位中,叶的黄酮类各组分含量远高于根、茎;百两金及其变种大叶百两金的山奈酚含量远高于其他种,其叶含量达6.42～9.13mg/g,可作为山奈酚资源植物加以利用.%Based on analyzing results of the active components flavonoid content in Sichuan Ardisia medicinal plants, to compare the flavonoid content differences among species and different part of the same plant; to explore the relativity of genetic relationship among species in Ardisia and the content of three types of flavonoid; and to offer some experimental evidences for establishing quality control standard of medical herbs from A rdisia. The HPLC was performed using Symmetry C18 column (4.6mm × 250mm, 5 μ m) and methanol-water (50:50) as the mobile phase. The flow rate was 0.8mL/min, the detection wavelength was 368nm, and the column temperature was 30 ℃.. The external standard method was used to carrying out quantitative analysis. The regression equation of myricetin, quercetin and kaempferol was separately Y=36 23IX-133 956,r=0.999 6; Y=45 758X-53 050=0.999 5; Y=27 569X-7 2635,r=0.999 6. The linear range was 0064~1.92a 0.084~2522j0.061~ 1.841 μ g respectively, the average recovery was 98.19% ,97.88% ,99.58% respectively. RSD was all less than 1.6%. And there are myricetin, quercetin and kaempferol in all these 7 species and 2 subspecies. Under the same condition, the amount of quercetin in plants of Ardisia sect. Crispardisia is higher than that of sect. Bladhia; the amount of myricetin, quercetin andkaempferol in leaves was much higher than those in stems and roots. The amount of kaempferol of A. Crispa and A. Crispa var. Amplifolia, which was up to 6.42～9.13mg/g, was much higher than all other species; so it is recommended that these two species should be used as the resource plants of kaempferol.