吉林不同产地哈蟆油中胆甾-5-烯-3-β-醇的含量测定及比较

摘要

Oviductus Ranae is also known as Oviductus Ranae,with strengthening the spleen and stomach,and nourishing the kidney functions.It is a traditional medicine which can cultivate immunity and enhance the body functions.To establish the HPLC method for the determination of cholest-5-ene-3-β-alcohol in Oviductus Ranae.To compare the content of cholest-5-ene-3-β-alcohol in the Oviductus Ranae collected from different areas of Jilin Province.Using HPLC column COSMOSIL Packed Column Cholester(4.6 mm× 250 mm,5μm);The mobile phase was consisted of acetonitrile-water,and it was used as gradient elution (the acetonitrile-water ratio was 85 ∶ 15 between 0min-28min,and acetonitrile ratio was 100％ between 28.01min-70min);The flow rate was 2.0mL/min,and the detection wavelength was 205ran;The column temperature was maintained at 35℃,and the injection volume was 20μL.The cholesten-5-ene-3-betaalcohols showed a good linearity in the range of 16.50βg/mL to 98.88βg / mL.The average recovery was 103.7％,and the RSD was 3.67％ (n=6).The method has the advantages of good reproducibility and high accuracy.It is convenient and feasible,and can determine the content of cholest-5-ene-3-β-alcohol in the Oviductus Ranae quickly and accurately.It can be used for the quality control of the Oviductus Ranae.%哈蟆油又称林蛙油,具有健脾养胃、滋阴补肾等功效,是一种扶正固本的药材.建立了哈蟆油中胆甾-5-烯-3-β-醇含量的HPLC测定方法,并比较吉林省不同产地哈蟆油中胆甾-5-烯-3-β-醇的含量.色谱柱:COSMOSILPackedColumnCholester(4.6mm×250.0mm,5μm);流动相:乙腈-水,采用梯度洗脱(0min～28min,V乙腈∶V水=85∶15;28.01min～70min,乙腈比例为100％);流速:2.0mL/min;检测波长:205nm;柱温:35℃;进样量:20μL.胆甾-5-烯-3-β-醇在16.50μg/mL～98.88μg/mL范围内呈良好线性关系,平均回收率为103.7％,RSD值为3.67％(n=6).本方法重复性良好,准确度较高,且方便可行,能够快速、精准地测定出哈蟆油中胆甾-5-烯-3-β-醇的含量,可用于哈蟆油的质量控制.