To establish in vitro culture system of abdominal fibroblast, ear fibroblast, testicular fibroblast, pulmonary fibroblast and splenic fibroblast, the newborn Bama mini pigs were taken as material, micro-liquid tissue block adhering wall method was used for primary culture and isolation of the above cells, routine subculture, freezing, and recovery procedure were performed; and their growth capacity and proliferation life span were observed in vitro according to cell morphology and growth characteristics. The results showed that the above fibroblasts were successfully isolated and could be used for passage and freezing; the cells could still be cultured and subcultured after freezing-thawing; abdominal fibroblasts immunofluorescence showed that vimentin expression was positive and cytokeratin was negative. The result of this study could provide different types of materials for the micromanipulation, hand - manipulation of nuclear transfer and transgenic related manipulations.%为建立广西巴马小型猪腹部成纤维细胞、耳成纤维细胞、睾丸成纤维细胞、肺部成纤维细胞及脾成纤维细胞体外培养体系,以新生巴马小型猪为试验材料,使用微量液体组织块贴壁法原代培养分离几种成纤维细胞,进行常规传代培养、冷冻和复苏；根据细胞的形态、生长特性等观察其体外生长能力和增殖寿命.结果显示,广西巴马小型猪腹部成纤维细胞、耳成纤维细胞、睾丸成纤维细胞、肺部成纤维细胞及脾成纤维细胞获得成功分离,体外可以传代和冷冻；冷冻复苏后仍可以正常培养和传代；腹部成纤维细胞免疫荧光检测结果显示波形蛋白表达阳性,角形蛋白表达阴性.研究成果可为显微操作核移植、手工核移植和转基因相关操作等提供不同类型的供体材料.
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