以凉粉草带茎节的茎段为外植体,采用I9(34)正交设计,对影响凉粉草组培快繁过程的3个因素(6 -BA、ZT、NAA)的3个浓度水平进行优化试验,试验结果采用SPSS13.0软件统计分析,并针对凉粉草在组培快繁过程中极易发生玻璃化等问题进行研究.结果表明:凉粉草的最适初代诱导培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,无玻璃化；最适继代增殖培养基为MS +6-BA0.5 mg/L +ZT0.5 mg/L+ NAA 0.02 mg/L+ PVA 1000 mg/L,无玻璃化,增殖系数达9.1,芽壮；最适生根培养基为1/2MS+NAA0.1 mg/L+KT0.01 mg/L.1000 mg/L PVA为有效防治凉粉草组培快繁过程中玻璃苗发生的最佳浓度.采用这一技术,理论上1株试管苗一年可以生产凉粉草种苗约为9万株.%Aseptic nodal stem segments being used as explants,three factors (6-BA,ZT,NAA) affecting the in vitro rapid propagation of Mesona chinensis Benth.were optimized under three concentration levels by L9 (34 ) orthogonal design.The results showed that the optimal media for primary induction,subculture and rooting of M.chinensis Benth.in vitro were respectively as follows:MS +6-BA 0.5 mg/L + NAA 0.1mg/L ( no vittrication),MS + 6-BA 0.5 mg/L + ZT 0.5 mg/L + NAA 0.02 mg/L + PVA 1000 mg/L (resulted with high multiplication coefficient by 9.1,strong buds and no vitrification),1/2MS + NAA0.1 mg/L + KT0.01 mg/L.The suitable concentration of PVA used to avoid effectively the vitrification of tube-seedlings in rapid propagation ofM.chinesis Benth.was 1000 mg/L.By using this technique,theoretically,90 000 young individuals of M.chinensis Benth.would be produced from only 1 tube-seedling in one year.All experimental data were analyzed using SPSS 13.0.In addition,seedling vitrification during in vitro propagation was discussed also.
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