广西是柑桔原产地之一,蕴藏着丰富的柑桔种质资源.选用8对AFLP引物组合,对42份广西地方柑桔资源和广西柑桔主栽品种进行遗传多样性分析及分类研究.共获得清晰可见标记377条,其中多态性标记245条,占65.0％.在遗传相似系数0.92处,可以将柑桔品种分为柚、橙、柑、桔、金柑5个类群.供试材料两两间遗传相似系数在0.75～1之间.根据遗传相似系数( UPGMA,N-J)得到的分类树状图与传统方法的分类结果相似.研究结果表明广西柑桔种质资源具有较高的遗传多样性,广西柚类品种两两间遗传相似系数在0.93 ～0.97之间,广西酸桔品种间亲缘关系较近.%Guangxi was one of original areas of citrus, and owned rich citrus germplasm resources. To analyze the genetic diversity of citrus varieties and classify the citrus varieties of Guangxi, 8 pairs of AFLP amplified primers combination were selected. 377 visible AFLP bands were observed ,of which 245 bands were polymorphic, accounting for 65.0 %. Citrus varieties could be classified into 5 taxa at which the genetic similarity coefficient was 0.92. Genetic similarity coefficient among the test material ranged from 0.75 to 1. 42. Phenetic tree based on genetic similarity coefficient (UPGMA, N-J) was similar to the taxa using traditional methods. The results showed that the genetic diversity of citrus germplasm resources was high in Guangxi, the genetic similarity coefficient between pummelo varieties ranged from 0.93 to 0.97, the genetic relationship among sweet oranges was very near.
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