藏红花愈伤组织的诱导及植株再生

摘要

以藏红花(Crocus sativus L.)当年新生小球茎为材料,在MS+0.5 mg/L BA+1.0 mg/L NAA+7%CM(椰乳)培养基上进行初代培养,再转入MS+5 mg/L BA+5 mg/L NAA中进行愈伤组织的诱导,黑暗条件下继代1-2次,51 d就可以获得高诱导串的愈伤组织,愈伤诱导率可达65%.将愈伤组织转入分化培养基MS+5 mg/L BA+5 mg/L NAA中分化丛生芽,丛生芽诱导率高达95%,将丛生芽分成单个植株,最终形成完整再生植株.%The corms of saffron were used as explanta to establiah a regeneration protocol for Cro cus sativus L. The corms incubated in MS ＋ 0. 5 mg/L BA ＋1.0 mg/L NAA ＋7 ％ CM at first, then tranaferred to MS ＋5 mg/ L BA ＋5 mg/ L NAA for callus induction. Callus formation was obaened after 51 days , culture and the callus induction rate was 65 ％ . After four weeks callus induction, the shoot regeneration rate (95 ％ ) was obtained when 2 mg/L BA was combined with 1 mg/L NAA. The shoot buds were fragmented and transfered to root induction medium containing 0.5 mg/L IBA. Plantlets were then successfully transplanted to greenhouse.