以箭根薯花器官不同部位为外植体,通过正交试验设计及随机试验分析了不同因素对箭根薯花器官愈伤组织诱导、增殖、分化、生根及组培苗移栽成活率的影响.结果表明,箭根薯雌雄蕊为其花器官诱导愈伤组织较好的部位,愈伤组织诱导与增殖最佳培养基为:MS+0.5 mg/L 6-BA +0.2 mg/L KT +2.0 mg/L2,4-D,箭根薯愈伤组织分化较好的培养基为:MS +2.0 ～3.0 mg/L6-BA,箭根薯组培苗生根较好培养基为:MS+ 0.3 mg/L NAA,箭根薯组培苗移栽较适合的基质为1/2火土+1/2细沙.%The explants were obtained from different sections of flower organs of Tacca chantrieri. The effects of different factors on callus induction of Tacca chantrieri, multiplication culture, differentiation of callus, rooting induction and survival rate of culture seeding were investigated using orthogonal design L9 (34 ) and random test. The results showed that the filaments of Tcacca chantrieri were the best explants on callus inducement. The best medium for the first generation callus inducement and the multiplication culture were MS +0.5 mg/L 6-BA + 0.2 mg/L KT + 2.0 mg/L 2,4-D, relatively better differentiation of callus medium was MS + 2.0 - 3.0 mg/L 6-BA, and relatively better rooting medium was MS +0.3 mg/L NAA. Proper matrix for transplant of Tacca chantrieri culture seedlings were 1/2 fine sand + 1/2 ash soil.
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