With the aim to investigate the possible correlation of homogluthathion synthesis in soybean with tolerances to phosphorus deficiency (-P) and/or aluminum toxicity ( + Al) , BX10, a dual tolerant cultivar to both stresses, was used as plant materials to analyze the expression of two key enzymeB γ-ECS and hGSHS. Soybean were cultured with 1/5 Hoagland nutrient and stressed by decreasing P from 0.2 mM to 0.2 μ,M and adding Al to an ultimate concentration of 0.5 mM, leaves and roots were harvested at 6 h, 2 d and 12 d after stress. The cDNA fragments of y-ECS and hGSHS genes were cloned and certified by sequencing, and their spatial and temporal expression under the P and Al stress were investigated using semi-quantitative RT-PCR with Lectin as reference gene. The results showed that the expressions of y-ECS and hCSH were induced by both P deficiency and Al toxicity, and the extents were progressively increased in the early stress durations (2 d) , while in the long term stress (12 d) , difference in expression patterns occurred between stresses and/or tissues. Based on the results , the possible mechanism including stress action, subcellular location of enzymes was discussed.%以磷高效、耐铝毒的大豆双抗品种BX10为实验材料,采用1/5Hoagland营养液水培并进行低磷(-P)和铝毒(+Al)胁迫处理,收集胁迫后6h、2d和12 d的根叶材料,克隆大豆γ-ECS和hGSHS基因cDNA片段并测序验证,以大豆凝集素Lectin基因为内参基因,用半定量RT-PCR技术检测胁迫条件下大豆根和叶片中γ-ECS和hGSHS基因的表达情况,结果表明:低磷和铝毒胁迫均诱导了两个基因的表达,前期(2 d)增加幅度随着胁迫时间延长而增加,但胁迫后期(12 d)两个基因的表达在不同胁迫处理和组织间表现出不同的变化趋势,这些差异可能与胁迫的作用特性和hGSH合成酶的细胞定位有关.
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