茶树组织培养中外植体褐化控制的研究

摘要

为了控制茶树外植体在组织培养过程中的褐化现象,从外植体表面消毒时间、抗氧化剂和吸附剂的使用、琼脂浓度和pH值的高低进行了综合性比较试验.结果表明:外植体表面消毒时间以6min为宜;外植体接种在MS +6-BA 2.0 mg/L+ NAA 0.2mg/L+GA31mg/L+核黄素0.02g/L的培养基上,琼脂浓度为5.0g/L,pH值为5.6,外植体生长状况良好,褐化率由57％下降为13.3 ％.%The sterilized time of the tea erplants, different antioxidants and adsorbents used, and different concentration of pH and agar were studied to reduce the browning rate of explants in tissue culture of tea tree. The results showed that the browning rate would decrease 57 % to 13.3 % when the surface disinfection suitable time lasted about 6min,and explants were inoculated on MS+6-BA 2.0 mg/L + NAA0. 2 mg/L + GA3 1mg/L + VB2 0. 02 g/L of medium, with agar concentration to 5.0g/L, pH of 5.6,and the growth of explains was in good condition.