In this paper, taken the maize inbred line of 18-599 as tested materials, the improved Hoaglands' nutritive medium without NH4+ was used to treat 18-599 seedlings without endosperm with different concentrations of nitrate which were replaced weekly as the only limiting factors. After 21 days, the maize growth and development traits of root numbers and dry weight, stem-leaf dry weight, leaf area, chlorophyll contents and so on were investigated, and the relations and interaction between them were analyzed with SPSS13.0 statistical software. The results showed that root numbers and dry weight were very significantly negative correlation with nitrate concentrations, and the coefficients were -0.926 and -0.934, respectively. Regression analysis indicated that the optimal NO3- concentration for maize inbred line of 18-599 seedling growth was 9.6 mol/L, which could promote the roots, stem-leaf growth,increase chlorophyll content, improve the corn seedling photosynthesis, and accumulate material so as to get high yield of corn.%本研究以玉米骨干自交系18-599为材料,采用改良霍格兰氏营养液配方,去除铵高子,不同浓度硝酸盐作为限制因子,处理发芽后去胚乳的玉米幼苗.21 d后,测定根条数、根干重、茎叶干重、叶面积以及叶绿素含量等玉米生长发育指标,利用SPSS13.0进行统计分析.结果表明,根条数、根干重与硝酸盐浓度呈板显著负相关,相关系数分别为-0.926和-0.934；回归分析显示玉米骨干自交系18-599苗期生长发育的最佳NO3-浓度为9.6 mol/L,促进根系生长,促进茎叶生长、提高叶面积指数和叶绿素含量,有利于光合作用,积累产量形成所需的大量物质从而促进后期玉米的高产和穗产.
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