江苏省瓜蒌根结线虫种类鉴定

摘要

本文对江苏瓜萎上根结线虫样本进行广泛采集,单卵块纯化培养后,运用形态学方法和分子生物学方法对其进行了鉴定.根据雌虫会阴花纹、雌虫、雄虫和2龄幼虫的相关测量值等形态学鉴定特征,并结合特异引物的PCR扩增图谱,鉴定出该病原为南方根结线虫(Meloidogyne incognita).结果表明,江苏省瓜萎根结线虫群体类型为南方根结线虫,将进一步为该病害传播、流行和控制提供可行的理论依据.%In this paper, Meloidagyne populations on Trichosanthes spp. were collected and purified in Jisngsu. Based on diagnostic characters and morphometrics of females, males, and second-stage juveniles, the perineal patterns of female, using molecular biological techniques , the root-knot nematodes isolated from Trichasanthes were identified as Meloidogyne incognita with morphology and molecular biology. The results showed that the identification of Trichosanthes spp. which occurred on in Jiangsu was Meloidogyne incognita,which would provid theoretical basis for control of the root-knot nematodes disease.