超高效液相色谱(UPLC)同时测定水性胶黏剂中甲醛、乙醛、丙烯醛

摘要

A method for separation and determimtion of formaldehyde,acetaldehyde and acrolein in water-borne adhesives was developed by ultra performance liquid chromatography (UPLC).Deionized water was used to extract water-borne adhesives.In acid conditions,three kinds of aldehyde was derived by 2,4-dinitrophenylhydrazine,and were separated on a ACOUITY UPLC (R) BEH C18 column(1.7 μn, 100 mm × 2.1 mm)column,using water-acetonitrile as mobile phase.Formaldehyde,Acetaldehyde and Acrolein in water-borne adhesives were separated within 8.0 minutes and then determined by UPLC with a diode array detector.The average recoveries of formaldehyde,acetaldehyde and acrolein were 100.66 ％,96.57 ％,99.56 ％,respectively,and the relative standard deviations were in the range of2.1％-3.4 ％.This is a faster,simpler and solvent-saving method,and is suitable for rapid analysis of formaldehyde,acetaldehyde and acrolein in water-borne adhesives.%建立了超高效液相色谱(UPLC)法同时测定水性胶黏剂中甲醛、乙醛和丙烯醛的分析方法.水性胶黏剂采用去离子水进行振荡萃取,在酸性条件下,经2,4-二硝基苯肼(DNPH)衍生化后,采用ACOUITY UPLC(R)BEH C18 2.1 mm×100 mm(1.7 μm)柱为分离柱,以乙腈-水为流动相,采用优化后的条件二极管阵列检测器进行检测,分析时间为8.0 min.结果表明,甲醛、乙醛、丙烯醛的回收率分别为:100.66％、96.57％、99.56％,相对标准偏差为2.1％～3.4％；该方法分析时间短、流动相消耗量少、结果准确可靠,适合于水性胶黏剂中甲醛、乙醛和丙烯醛的快速测定.