家兔ANGPTL4基因多态性及其与生长性状的关联性分析

摘要

[目的]探究家兔血管生成素样蛋白4(angiopoietin-like protein 4,ANGPL4)基因第6外显子内的多态性及其与家兔部分生长性状的关联性.[方法]采用PCR产物测序法检测家兔ANGPTL4基因外显子内的单核苷酸多态性(SNPs)位点,并应用PCR-RFLP酶切分型技术对3个家兔品种(天府黑兔、伊拉兔和新西兰兔)共495个群体样本进行酶切分型,分析SNPs与3个家兔品种部分生长性状的关联性.[结果]ANGPTL4基因第6外显子中共检测发现3个遗传变异位点(c.823 G＞A为非同义突变,c.867 T＞C和c.975 T＞C均为同义突变),且完全处于连锁状态(r2≥0.75),由此选择c.823 G＞A位点作为遗传效应分析位点.关联性分析得出3个家兔群体内AG基因型和GG基因型与3个品种部分生长性状具有较高的遗传效应.[结论]ANPGTL4基因可以是与家兔生长发育相关的候选基因,以此为培育肉兔新品种提供辅助分子遗传选择依据.%[Objective] The aim of this study was to investigate the association between polymorphisms in the angiopoietin like protein 4 (ANGPTL4) gene and growth traits of domestic rabbits.[Method] The polymerase chain reaction (PCR) products were first screened for single nucleotide polymorphisms (SNPs) of rabbit ANGPTL4 gene by direct DNA sequencing,and the PCR-restriction fragment length polymorphism (RFLP) assay was further used for genotyping of a total of 459 samples from three meat rabbit breeds (Tianfu Black,.IRA and New Zealand rabbits).Thereafter,the association of polymorphisms in this gene with growth traits was evaluated.[Result] Our results showed that three SNPs,including a non-synonymous mutation c.823 G ＞ A and two synonymous mutations c.867 T ＞ C,and c.975 T ＞ C,were detected in exon 6 of ANGPTL4.Owing to a completely linkage relationship (r2 ≥ 0.75) among these three SNPs,only the c.823 G ＞ A was selected for analysis of genetic effects.The association analysis of its genotypes with growth traits revealed that the genotypes of AG and GG were strongly correlated with some growth traits in three breeds of rabbits.[Conclusion] These data suggested that ANPGTL4 could be a candidate gene affecting rabbit growth and the SNP c.823 G ＞ A might be used as a potential genetic marker in rabbit breeding.