[目的]为了在不影响后代生产性能的基础上最大限度地提高体内、体外胚胎移植效率,从而为体外胚胎在生产中的应用奠定基础.[方法]从移植胚胎数量方面着手,比较其对体内、体外胚胎移植受胎率和产羔率的影响;并以常用的胚胎移植作为对照,研究体外胚胎与其生产效率存在的差距;并对不同来源的后代生产性能进行测定,对比体外胚胎在生产中的生产效率.[结果]①移植2枚体外早期胚胎的受胎率(60.42％)显著高于移植1枚胚胎的结果(48.54％)(P＜0.05),前者的产羔率也极显著高于后者(74.68％vs41.96％,P＜0.01);②移植单枚体外胚胎的受胎率和产羔率皆显著低于体内胚胎(P＜0.05);③受体移植双枚体内或体外胚胎,受胎率和产羔率都没有差异(P＞0.05).④体外胚胎移植后代的公羔比例与体内胚胎没有差异,但显著高于自然繁殖(P＜0.05).⑤体外胚胎的后代虽然初生重要显著低于体内胚胎(4.98 vs6.2,P＜0.01),但其3月龄体重和存活率与体内胚胎后代皆没有差异(P＞0.05),且与自然繁殖组间都没有差异(P＞0.05).[结论]相对于早期胚胎,体外胚胎移植2枚,体内胚胎移植1枚的生产效率较高.并且对后代生产性能没有影响.%[Objective] In order to improve the efficiency of in vivo and in vitro embryo transfer,the effects of in vitro and in vivo embryo transfer and the rate of lambing were compared with the number of embryos.[Method]The differences between the in vitro embryonic and in vivo embryo transfer productivity were also studied.[Result] The results were as follows:(i) The pregnancy rate of two early embryos in vitro was significantly higher than that of single embryos (60.42 ％ vs 48.54 ％,P ＜ 0.05);The former lambing rate is also significantly higher than the latter (74.68 ％ vs 41.96 ％,P ＜ 0.01).(ii) The rate of pregnancy and lambing rate of single in vitro embryos were significantly lower than that of in vivo embryos (P ＜ 0.05).(iii) There was no difference in conception rate and lambing rate between two in vivo embryos or in vitro embryos (P ＞0.05).(iv) There is no difference between male and female embryos in the ratio of male lambs to embryos in vitro (P ＞ 0.05);But it is significantly higher than that of natural reproduction (P ＜ 0.05).(v) Although the offspring birth weight of in vitro embryos was significantly lower than that of in vivo embryos (4.98 vs 6.2,P ＜ 0.01),the body weight and survival rate at 3 months of age did not differ from those of embryos in vivo (P ＞ 0.05).And there was no difference with the natural breeding group(P ＞ 0.05).[Conclu sion] Based on the above results,the highest efficiency was achieved when the two early embryos in vitro or one early embryo in vivo were transplanted.
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