毛果杨CBF/DREB1基因家族生物信息学分析

摘要

[目的]为了对毛果杨CBF基因家族进行系统分析,利用毛果杨基因组数据库,通过生物信息学的方法,鉴定毛果杨CBF基因家族的染色体定位、编码蛋白和磷酸化位点预测.[方法]通过序列比对进行进化和分类分析.[结果]毛果杨含有6个CBF基因,均不含内显子,分布于毛果杨的5条染色体上.[结论]MEME保守基序分析显示,杨树CBF蛋白均含有2个保守的基序,他们共同构成AP2结构域.磷酸化位点预测分析表明毛果杨CBF蛋白均含有大量的磷酸化位点.以上结果将为今后揭示毛果杨CBF蛋白的功能提供重要的线索.%[Objective] The study aims to analyze systematically CBF gene family in Populus trichocarpa.[Method] On the basis of P.trichocarpa genome database and bioinformatics method,we obtained P.trichocarpa CBF gene structure,position on chromosome,proteins and phosphorylation site.[Result] Six PtCBF genes existed in the P.trichocarpa genome and located on 5 different chromosomes.[Conclusion] Using MEME method,multiple alignment and motif display results indicated that all PtCBF proteins contain two conserved motifs,constituted AP2 domain.Phylogenetic analysis revealed that the PtCBF proteins could be divided into 3 groups.Phosphorylation site prediction of PtCBF proteins showed that there were many phosphorylation sites.These results would provide the important clues for functional studies to reveal the role of CBF/DREB1 gene family in P.trichocarpa.